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. Author manuscript; available in PMC: 2018 Dec 1.
Published in final edited form as: Dev Biol. 2017 Oct 3;432(1):178–191. doi: 10.1016/j.ydbio.2017.09.038

Figure 2. Motor exit points shift ventrally in Robo or Slit mutants.

Figure 2

(A–D) βIII-tubulin labeling on spinal cord sections showing motor exit points shift closer to the floor plate in Robo1−/−;2−/− or Slit1−/−;2−/− mutants. Robo1+/−;2+/− (n=8 embryos), Robo1−/−;2−/− (n=10 embryos), Slit1−/−;2+/− (n=6 embryos) and Slit1−/−;2−/− (n=10 embryos). (E) Schematics showing how the measurements are examined in control and Robo1−/−;2−/−. The distance from the midline to the most ventral exit point (dmv, solid and dotted blue lines) is normalized by the height (dh)of each embryo (dnv = dmv/dh). (F, G) Summary graphs show the distance from the midline to the most ventral exit point (F), and the distance from the midline to the average position of all of the exiting motor axon bundles (G) in Robo1−/−;2−/− or Slit1−/−;2−/− mutants compared to their littermate controls. Both distances are significantly decreased in Robo or Slit mutants compared to their littermate controls. Yellow arrow heads in A–D show the closest and farthest exit points from the ventral midline. Dotted yellow lines in A–D show the floor plate. Scale bars: A–D, 50 μm. *= P < 0.01, ** = P < 0.001.