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. Author manuscript; available in PMC: 2018 Dec 1.
Published in final edited form as: Dev Biol. 2017 Oct 3;432(1):178–191. doi: 10.1016/j.ydbio.2017.09.038

Figure 7. Spinal motor nerve projections are abnormal in Netrin-1 mutants.

Figure 7

(A–F) Spinal cord sections of Netrin-1+/+::Islet-1MN-GFP-F and Netrin-1−/−::Islet-1MN-GFP-F embryos (n=6 embryos for each genotype of E12.5; note that these examples appear slightly less advanced than in Fig 4) show that motor trajectories are widely dispersed at the exit points and at the choice points in Netrin-1−/−::Islet-1MN-GFP-F embryos. Dotted white lines in A, B, D, and E show the spinal cord. Yellow arrows in B and E show motor axon trajectories at exit points. (G) Quantification of the thickness at exit points (dotted blue lines in B and E) shows that Netrin-1 mutants have defasciculated exit points compared to their controls. (H) Quantification of the thickness at choice points (dotted red lines in C and F) shows enhanced defasciculation within the choice point, leading to extensive ventral ramus in Netrin-1 mutants. Scale bars: A, D, 100 μm; B, C, E, F, 50 μm. SC, spinal cord; DM, dermomyotome. *=P < 0.05.