Figure 2. Granule cell precursor-specific loss of Lkb1 results in increased foliation and cortical expansion.

A–A′. Hematoxylin and eosin staining of control (A) and Lkb1^cko (A′) cerebella at P30. Roman numerals denote lobule numbers. Red roman numerals indicate lobules present in Lkb1^cko absent in control. B. Average lobule number of control and Lkb1^cko cerebella. C–E. Hematoxylin and eosin staining of mid-vermal cerebellar cross sections at the indicated stages. Lobules present in Lkb1^cko not present in the control are highlighted in red in C″–E″. Asterisks in C–C′ indicate cardinal fissures. F. Average cross sectional area of mid-vermal cerebellar sections at indicated stages. G. Average cross sectional perimeter of mid-vermal cerebellar cross sections at the indicated stages. H. Illustration showing how area and perimeter were determined. I–I′. Whole mount images of P6 control (I) and Lkb1^cko (I′) cerebella. Dashed lines delineate folia. n=5 for all analyses. *, p<0.05, **, p<0.005, ***, p<0.0005, Student’s t-test. Scalebar 500 μm for all images. Con = control.