Figure 3. Hedgehog signaling is unchanged in Lkb1^cko cerebella.

A–B. In situ hybridization for Hedgehog target gene Gli1 in postnatal day 6 control (A–A′) and Lkb1^cko (B–B′) cerebella. C. Western blot for Gli1 in GCPs derived from control and Lkb1^cko cerebella. Lkb1 and Actin were used to verify knockdown and loading, respectively. D. Densitometric measurements of Gli1 protein levels relative to Actin. E. Quantitative real-time PCR for Gli1, Ptch1, and Hhip1 in freshly isolated control and Lkb1^cko GCPs. n=3, no significant difference, Student’s t-test. F. Western blot for Gli1 in cultured GCPs derived from control and Lkb1^cko cerebella in the presence (+) or absence (−) of 20nm SAG. G. Western blot for Gli1 in whole cerebella derived from control and Gli1^−/− mutants. Scalebar 50 μm. Con = control, EGL = external granule layer. Full-length blots are shown in Supplementary Figure 9.