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. 2001 Jul 31;98(18):10232–10237. doi: 10.1073/pnas.171243598

Figure 3.

Figure 3

Effects of TsixEF-1α on Xist expression under differentiation conditions. (A) Quantitative RT-PCR for Xist expression in wild-type (16.7) and mutant (H12) cells placed under differentiation conditions for the number of days shown. Xist levels were normalized to Rpo2–1 levels; fold Xist induction reflects the change from day 0. (B) Allele-specific Xist RT-PCR of wild-type (16.7) and mutant (2B1) cells. Amplification was carried out with primers F and H (spanning Xist introns, as shown in Fig. 2A) and polymorphic ScrFI fragments detected with G.