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. 2017 Nov 14;11:352. doi: 10.3389/fncel.2017.00352

Figure 2.

Figure 2

Adiponectin deficiency leads to microglia hyper-responsiveness toward LPS. (A) Representative images of Iba-1-immunoreactive microglia (green fluorescence) in hippocampus (left panels) and hypothalamus (right panels) from Wt (upper panels) and ApN−/− (lower panels) mouse brains, 24 h after LPS challenge. Nuclei are stained with Hoechst fluorescent dye (blue fluorescence). All images were taken under the same parameters. (B) Quantification of Iba-1 staining using fluorescence intensity values to quantify Iba-1 levels. (C) Schematic representation of the protocol. 24 h after LPS ip injection, IL-1β, IL-6, and TNFα proteins (D) or mRNAs (E) were quantified by CBA or qRT-PCR, respectively, from microglia sorted from brain cell suspensions of Wt (white bars) or ApN−/− mice (black bars) cultured for 15 h. (D) Data represent the mean concentration ± SEM, n = 4. (E) Data represent the mean expression levels ± s expressed as fold change compared to LPS-treated Wt mouse microglia, n = 4. Mann & Whitney for comparison between groups, *p < 0.05, vs. control.