Figure 5.

Globular adiponectin limits LPS-induced oxidative and nitrosative stress induction. BV2 cells were pre-treated for 1 h with saline (white and black bars) or gApN (gray bars) before addition of saline (white and dark gray bars) or LPS (black and light gray bars) for 3 (C,D) or 15 h (A,B). Reactive oxygen species (ROS) (A) and nitric oxide (NO) (B) were quantified by flow cytometry and Griess reagent assay, respectively, as described in the Materials and Methods section. N = 4–6 per group; Kruskal-Wallis test followed by the Iman-Conover method for multiple comparison between groups was performed, ^***p < 0.005. (C) Representative images of CD11b (green fluorescence) and iNOS (red fluorescence) immunoreactive BV2 cells pre-treated with saline (upper panels) or gApN (lower panels) and treated with saline (left panels) or LPS (right panels). (D) Quantification of CD11b and iNOS staining using fluorescence intensity values to quantify their respective expression level. N = 6 per group, Kruskal–Wallis test followed by the Iman-Conover method for multiple comparison between groups was performed, ^*p < 0.05, ^**p < 0.01.