Figure 6.

Globular adiponectin activates AMPK phosphorylation and limits NF-κB nuclear translocation. (A) Representative western-blot analysis (left panels) of AMPK phosphorylation at Thr172 (pAMPK) in BV2 cells. Right panel shows quantification by densitometry of pAMPK/AMPK ratio of western-blots from 3 independent experiments. Actin immunoreactivity was used for normalization. Mann & Whitney for comparison between groups, ^*p < 0.05, ^***p < 0.005. (B) Nuclear fractions were obtained from BV2 cells as described in the Materials and Methods section. Left panels show a representative western-blot of the p65 subunit of NF-κB and Histone H3 as a nuclear marker. Right panel shows quantification by densitometry of nuclear p65 immunoreactivity of western-blots from 3 independent experiments. Histone H3 immunoreactivity was used for normalization. Mann & Whitney for comparison between groups, ^*p < 0.05.