Table 1.

Effect of AdipoRon treatment on cytokine concentration in brain areas of Ctrl or LPS-injected mice.

pg/mg of protein	IL-1β				IL-6				TNFα
AdipoRon	–	+	–	+	–	+	–	+	–	+	–	+
LPS	–	–	–	–	+	+	+	+	–	–	+	+
Prefrontal cortex	0.4 ±0.04	0.4 ± 0.06	1.6 ± 0.2	2.1 ± 0.1	8.8 ± 2.1	8.1 ±1.0	1.4 ± 0.2	1.55 ± 0.2	0.05 ± 0.01	0.07 ± 0.03	0.4 ±0.05	0.5 ± 0.05
Hypothalamus	0.7 ±0.2	0.7 ± 0.3	2.6 ± 0.7	2.9 ± 0.8	11.8 ± 2.2	14.3 ±3.5	1.5 ± 0.2	1.8 ± 0.5	0.16 ± 0.09	0.3 ± 0.1	0.47 ±0.1	0.65 ± 0.08
Hippocampus	0.3 ±0.03	0.3 ± 0.04	1.7 ± 0.3	2.4 ± 0.2	9.2 ± 1.5	9.9 ±1.7	1.2 ± 0.1	1.9 ± 0.4	0.03 ± 0.01	0.03 ± 0.02	0.55 ±0.08	0.6 ± 0.04

Wt mice (10 weeks old) were daily i.p. injected with vehicle (2.5% DMSO solution) or AdipoRon (1 mg/kg in 2.5% DMSO solution) during 15 consecutive days. On day 15, mice received a single i.p. injection of saline or LPS (0.8mg/kg). Twenty-four hours later, mice received a last injection of AdipoRon and were sacrificed. Brains were dissected and pro-inflammatory cytokines were measured by MSD in different brain regions. Data represent means ± sem, n = 5 mice for LPS treated group without AdipoRon and n = 8 for LPS + AdipoRon treated group. A two-way ANOVA comparing the effect of AdipoRon in untreated and treated LPS groups showed no significant interaction while the effect of LPS was significant in all considered brain regions