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. 2017 Nov 14;8:1508. doi: 10.3389/fimmu.2017.01508

Figure 3.

Figure 3

Insufficiency in mediating in vitro antigen-specific immune suppression of interleukin-4 (IL-4)-deficient regulatory T cells (Tregs). (A) Schematic diagram illustrating the protocol for enriching OVA-specific Tregs from WT and IL-4 knockout (KO) mice. (B) The percentages of Foxp3 expressing cells among CD4+CD25+ T cells in OVA-immunized WT (n = 13) and IL-4 KO (n = 12) mice were analyzed by flow cytometry. (C) The suppressive capability of IL-4 KO and WT Tregs in exerting antigen-specific suppression. (D) The suppressive capability of IL-4 KO Tregs in suppressing WT responder T cells (Tresps). (E) The suppressive capability of WT Tregs in suppressing IL-4 KO Tresps. Results are presented as the relative percentage of cell proliferation to OT2 Tresps alone (C), WT Tresps alone (D), and IL-4 KO Tresps alone (E). Data are representative of two (B,C,E) and three (D) independent experiments (*p < 0.05; **p < 0.01; and ***p < 0.001).