Figure 6.

Physiological analysis of high expression lines of RPL23A. (A) The Δ¹³C measured in two high, L23A-15.8 and L23A-12.6, and two low expression, L23A-5.23, L23A-1.2 lines in T₁ generation had values of 19.24, 19.14, 21.5, and 20.9‰, respectively with respect to NS which had 23.75‰. (B) The chlorophyll fluorescence of two selected lines in the T₂ generation, performed with MINI-PAM had 0.86 and 0.88 under normal conditions, whereas under limited water conditions, both the lines showed Fv/Fm of 0.80. (C,D) The T₃ transgenic seedlings showed increased growth parameters at seedling stage under normal and treated conditions with respect to NC. The primary root was considered to calculate the root length using a 1 cm scale bar. (E) Proline content of T₃ transgenic and NC seedlings with and without PEG and NaCl treatments. (F–H) Chlorophyll measurements in the T₃ generation transgenic seedlings. The seedlings exhibited high levels of (A,B) and total chlorophyll contents with respect to NC. Mean values of chlorophyll data with ± standard error represented with asterisks were considered statistically significant at P < 0.05.