Figure 4.

Clustal W alignments of Mlph and three proteins that share a conserved Rab effector domain. The eight highly conserved Cys residues that make up the Zn²⁺ finger domain are shown in red. Identical and conserved amino acid residues are in shaded boxes. The seven amino acids deleted in leaden are shown in blue. The 6-aa aromatic-rich motif that binds to Rab is shown in yellow. Amino acids 1–129 of Mlph show 28% identity/48% similarity to granuphilin, 31% identity/49% similarity to Slp3-a, and 23% identity/41% similarity to rabphilin-3A.