Fig. 6.

Nuclear translocation of AR induced by DHT in OE33-AR in direct co-culture with fibroblasts. Fluorescent micrographs of OE33-AR grown for 6 days in monoculture or direct co-culture with PShTerts with vehicle or 10, 100, or 1000 nM DHT, or in direct co-culture with NFFs with vehicle or 10 nM DHT. Cells were labeled with rabbit antihuman AR polyclonal IgG (clone N-20; 1 µg/mL in 1.5% goat serum) followed by secondary antibody Alexa Fluor 568 goat anti-rabbit IgG (2 µg/mL in 1.5% goat serum) and DAPI (1 µg/mL). Merged channel images were captured using a Zeiss confocal LSM 700 microscope. Scale bar is 75 µM