Fig. 7.

Effect of co-culture with fibroblasts on DHT induced expression of androgen-responsive genes in OE33-AR. a The expression of FKBP5, HMOX1, and NDRG1 in OE33-AR grown for 6 days with vehicle or 10 nM DHT in monoculture or direct co-culture with NFFs or PShTert myofibroblasts. Expression was normalized to the reference gene ACTB and graphed relative to expression with vehicle. Data are the mean ± SD. b Expression of FKBP5 in OE33-AR grown for 6 days with vehicle or 10 nM DHT, with or without 15 µM enzalutamide, in monoculture or direct co-culture with PShTert myofibroblasts