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. 2017 Nov 14;8:929. doi: 10.3389/fphys.2017.00929

Table 1.

Primer sequences used for the relative quantification of the transcripts by qRT-PCR.

Transcript Primer sequences Length (bases) Amplicon size (bp)
Alkaline phosphatase (AlpI) CCTTGACTGTGGTTACTGCT 20 216
CCTGGTAGTTGTTGTGAGCG 20
Bone morphogenic protein 2 (Bmp2) CAGTAGTTTCCAGCACCGAA 20 199
CACTTCCACCACAAACCCAT 20
Bone morphogenic protein 4 (Bmp4) AGGAAGGAGTAGATGTGAGAG 21 158
AGGGACGGAGACCAGATAC 19
Bone carboxy-glutamic acid containing protein (Bglap) GCAGGAGGGCAATAAGGTAG 20 159
ATGCGTTTGTAGGCGGTC 18
CyclinD1 (Ccnd1) ACCCTGACACCAATCTCCTC 20 214
AAGCGGTCCAGGTAGTTCAT 20
Sp7 transcription factor (Sp7) CACAAAGAAGCCATACGCTG 20 165
CCAGGAAATGAGTGAGGGAAG 21
Runt-related transcription factor 2 (Runx2) TGCCTCCGCTGTTATGAAAA 20 187
CTGTCTGTGCCTTCTTGGTT 20