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. 2017 Nov 14;7:469. doi: 10.3389/fcimb.2017.00469

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Copyright © 2017 Boesze-Battaglia, Walker, Dhingra, Kandror, Tang and Shenker.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Extracted ion chromatograms of four representative doubly charged SYNGR2 peptides. Jurkat cells were treated with Cdt holotoxin as described in Materials and Methods for 1 h. Cell extracts were prepared and immunoprecipitated with immobilized anti-CdtB or isotype control IgG and the samples analyzed as described. The immunoprecipitates obtained with anti-CdtB mAb show strong signals for the peptides indicated; results are consistent with detection of MS/MS spectra for SYNGR2 peptides obtained exclusively with this mAb and were not detectable in the control immunoprecipitate.