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Immunoprecipitation of cellugyrin and CdtB in HPBMC and HeLa cells. HPBMC and HeLa cells were treated with medium or Cdt (2 μg/ml) for 2 h. Cell extracts were prepared as described in Materials and Methods and immunoprecipitated with immobilized control IgG or anti-CdtB mAb. The bound material was eluted and further analyzed by Western Blot for CdtB and cellugyrin. Results are representative of three experiments.
