Figure 8.

Comparative toxic effects of Cdt on Jurkat^WT and Jurkat^Cg− cells. (A) Shows the effect of Cdt on cell cycle arrest; Jurkat^WT and Jurkat^Cg− cells were incubated for 16 h in the presence of 0–5 pg/ml Cdt. Cells were stained with propidium iodide and cell cycle analysis performed using flow cytometry. The percentage of G2 cells is shown as a mean ± SEM for three experiments each performed in triplicate; solid bars represent Jurkat^WT cells and hashed bars Jurkat^Cg− cells. (B) Shows the effect of Cdt on apoptosis; cells were treated with 0–25 pg/ml Cdt for 48 h analyzed for DNA strand breaks using the TUNEL assay. Results are expressed as the mean percentage of TUNEL positive cells ± SEM for three experiments (); solid bars represent Jurkat^WT cells and hashed bars Jurkat^Cg− cells. ^*Indicates statistical significance (p < 0.05) when compared to untreated cells; ^**indicates statistical significance of p < 0.01.