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. 2017 Nov 14;8:903. doi: 10.3389/fphys.2017.00903

Figure 2.

Figure 2

Kir2.1 and Nav1.5 channels closely interact at the cell membrane. (A–D) IKir2.1 (A,C) and INav1.5 (B,D) traces recorded by applying the protocols shown at the top in CHO cells expressing Kir2.1 or Nav1.5 channels alone (A,B) or together (C,D) in control conditions (solid lines) or after dialyzing the cell with the antibodies indicated (dashed lines). (E–G) Normalized IKir2.1 (measured at −120 mV) (E,G) and peak INav1.5 (F,G) measured in the absence and presence of anti-Kir2.1 or anti-Nav1.5 antibodies in cells transfected with Kir2.1 or Nav1.5 channels alone (E,F) or cotransfected with both (G) plotted as a function of time. (H) Bar graphs showing the mean normalized current density values measured in each experimental group at the end of the recordings. Each point/bar represents the mean ± SEM of (n) cells of ≥ 3 preparations. In (H), *P < 0.05 vs. IKir2.1 or INav1.5 generated by Kir2.1 or Nav1.5 channels alone in the presence of anti-Nav1.5 or anti-Kir2.1 antibodies. For clarity the results of other statistical comparisons were not shown.