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Changes of serum antibody titers in chickens. Chickens in six groups were vaccinated with TPPPS adjuvant VP2–VP5–Fc vaccine (I), pure VP2–VP5–Fc vaccine (II), pure VP2–Fc vaccine (III), pure VP2 vaccine (IV), commercial VP2 subunit vaccine (V), and PBS (VI) at 7 days old, respectively. Serum was collected at 0, 7, 14, 21, 28, 35, 42, and 49 dpv. Then the antibody titers were determined by indirect ELISA. All values shown are means ± SD from three independent experiments.
