Figure 3.

Changes of cytokines in chickens. Chickens in six groups were vaccinated with TPPPS adjuvant VP2–VP5–Fc vaccine (I), pure VP2–VP5–Fc vaccine (II), pure VP2–Fc vaccine (III), pure VP2 vaccine (IV), commercial subunit vaccine (V) and PBS (VI) at 7 days old, respectively. Serum was collected at 0, 7, 14, 21, 28, 35, 42, and 49 dpv. Then IL-2 (A), IL-4 (B), and IFN-γ (C) were detected by using the chicken IL-2, IL-4, and IFN-γ ELISA kits. All values shown are means ± SD from three independent experiments.