. 2017 Nov 14;8:2258. doi: 10.3389/fmicb.2017.02258

Table 2.

Changes of CD4⁺ T lymphocyte counts in the peripheral blood.

Group^A	Days post–vaccination^B
	0	7	14	21	28	35	42	49
I	19.30 ± 0.04^ab	22.65 ± 0.80^ab	26.39 ± 0.65^a	29.62 ± 0.21^a	31.44 ± 0.50^a	37.04 ± 0.49^a	32.41 ± 1.02^a	31.40 ± 0.99^a
II	19.91 ± 0.58^a	23.31 ± 0.75^a	23.63 ± 0.85^b	25.24 ± 0.70^b	26.38 ± 0.58^b	31.09 ± 1.27^b	29.95 ± 0.84^b	28.81 ± 1.23^b
III	19.25 ± 0.88^ab	21.45 ± 1.09^ab	21.83 ± 0.53^bc	22.96 ± 0.83^c	23.99 ± 0.37^c	25.59 ± 0.54^c	24.64 ± 0.64^c	24.26 ± 0.67^c
IV	18.39 ± 0.79^b	20.74 ± 0.43^bc	22.66 ± 0.88^cd	21.25 ± 0.11^de	21.74 ± 1.10^d	23.09 ± 0.84^d	22.40 ± 0.35^d	22.24 ± 0.62^d
V	18.27 ± 0.23^b	19.01 ± 1.77^c	20.90 ± 1.10^d	22.03 ± 0.17^d	22.00 ± 0.68^d	22.95 ± 0.94^d	21.45 ± 0.78^d	20.90 ± 0.31^de
VI	19.59 ± 0.43^a	20.78 ± 1.96^bc	20.67 ± 1.03^d	20.79 ± 0.37^e	20.47 ± 0.31^e	20.75 ± 0.80^e	20.94 ± 0.17^e	20.68 ± 0.41^e

Group names represent that chickens in these groups were vaccinated with TPPPS adjuvant VP2–VP5–Fc vaccine (I), pure VP2–VP5–Fc vaccine (II), pure VP2–Fc vaccine (III), pure VP2 vaccine (IV), commercial VP2 subunit vaccine (V), and PBS (VI) at 7 days old, respectively.

CD4⁺ T lymphocyte counts in the same column were compared by Duncan's multiple–range tests. Different lowercase letter superscripts indicate significant differences (P < 0.05). Data are expressed as mean percentage ± SD.