Table 3.
qRT-PCR results of different sets of primers (ΔCT) and absolute numbers
| Sample ID | qPCR-set1 (ΔCT) | qPCR-set2 (ΔCT) | qPCR-set3 (ΔCT) | qPCR-Universal (ΔCT) | 16S rRNA copies | STD curve control |
|---|---|---|---|---|---|---|
| 1 | 28.6 | Undetermined | Undetermined | 25.8 | 15017 | Undetermined |
| 2 | 31.7 | Undetermined | Undetermined | 29.3 | 0 | Undetermined |
| 3 | 27.6 | 28.4 | 25.1 | 15.3 | 168 | Undetermined |
| 4 | 28.9 | 26.1 | 29.9 | 31.6 | 174 | Undetermined |
| 5 | 36.9 | Undetermined | Undetermined | 29.8 | 5 | Undetermined |
| 6 | 31.2 | Undetermined | Undetermined | 29.4 | 270 | Undetermined |
| 7 | 33.4 | Undetermined | Undetermined | 29.7 | 50 | Undetermined |
| 8 | 36.6 | Undetermined | Undetermined | 29.6 | 86 | Undetermined |
| 9 | 28.8 | Undetermined | Undetermined | 30.4 | 2366 | Undetermined |
| 10 | 19.6 | Undetermined | Undetermined | 24.4 | 14567 | Undetermined |
| 11 | 31.9 | Undetermined | Undetermined | 25.3 | 5558 | Undetermined |
| 12 | 32.5 | Undetermined | Undetermined | 29.7 | 148 | Undetermined |
| 13 | 29.6 | Undetermined | Undetermined | 28.9 | 157 | Undetermined |