Table 4.
Assessment of V1V2 dependent neutralization activity of 2B10 and 2E4 scFvs.
| IC50 Titers (μg/ml) |
Fold change |
||||||||
|---|---|---|---|---|---|---|---|---|---|
| scFv ID | 16055 WT | N156K | N160K | K169A | K171A | N156K | N160K | K169A | K171A |
| 2B10 | 35.5 | 34.3 | 33.78 | 32.4 | 34.9 | 0.96 | 0.95 | 0.91 | 0.98 |
| 2E4 | 81.2 | 80.3 | 80.5 | 79.6 | 74.6 | 0.98 | 0.99 | 0.98 | 0.91 |
The neutralization reactivity with V1V2 region mutants of the 2B10 and 2E4 scFvs were performed using HIV-1 subtype C pseudovirus 16055 and its V1V2 mutants N156K, N160K, K169E, and K171A using a starting concentration of 100 µg/ml of scFvs. Neither of the two scFv monoclonals shows V1V2-dependent neutralization activity.