Figure 1.

HLA-binding properties of neopeptides. Predicted eluted ligand likelihood percentile rank (EL%Rank) score of neopeptides, corresponding to individual studies (A) or summarized according to mutant peptide T cell response (B). As there is an overlap in patients between the Bentzen and the McGrannahan study, only unique observations are plotted, the first refereeing to the peptides tested with barcode labeled multimers and the second with fluorescently labeled tetramers (A). (C) Predicted EL%Rank score for neopeptides and their corresponding normal peptides, with mutant peptide T cell response and anchor position mutations indicated. The curve corresponding to the median EL%Rank_n/EL%Rank_m value equal to 1.2, used to define the groups of peptides with improved binding strength (IB) and conserved binding strength (CB), is shown as a solid line. Thresholds for weak (2 EL%Rank) and strong binders (0.5 EL%Rank) are indicated with dashed lines. ****P < 0.0001.