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Effect of Wnt4 on chicken granulosa cell (GC) proliferation. (A) Overexpression of Wnt4 in GCs stimulated cell proliferation, as determined by the CCK8 method. (B) Knockdown of Wnt4 in GCs inhibited cell proliferation, as determined by the CCK8 method. (*p < 0.05, **p < 0.01, ***p ≤ 0.001).
