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. 2017 Nov 15;8:1529. doi: 10.3389/fimmu.2017.01529

Figure 2.

Figure 2

P2X7 receptor (P2X7R) is present on monocytes in blood vessels of secondary progressive multiple sclerosis (SPMS) frontal cortex. (A) Immunohistochemistry on sections from human frontal cortex shows roundish P2X7R-positive cells distributed within distinct clusters throughout the entire SPMS tissue. Confocal double immunofluorescence analysis of these clusters exhibits colocalization of P2X7R protein (red) with CD45 leukocyte marker [(B), green]. Staining with Lectin from Lycopersicon esculentum for vascular endothelial cells [(C,D), green] clearly shows the presence of P2X7R-positive roundish cells (red) within the lumen of blood vessels (asterisk). Double immunofluorescence of P2X7R-positive clusters shows colocalization of P2X7R (red) with cluster of differentiation 14 (CD14) monocyte/macrophage marker [(E), green]. Confocal triple immunofluorescence analysis is then performed with antibodies for CD14 [(F,G), green], P2X7R [(F,G), red], and microglia/macrophages markers CD68 (F) or major histocompatibility complex II [(G), blue]. The asterisk always indicates the lumen of blood vessels, arrows indicate P2X7R-negative cells, and arrowheads P2X7R-positive cells.