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. 2017 Nov 20;8:1610. doi: 10.1038/s41467-017-01732-9

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 3 — DNA in the crXNA molecule affects target-binding affinities. a Nucleoside composition of AAVS1 crXNA molecules; RNA shown in lowercase red, DNA in uppercase black. b BLI sensogram showing association kinetics to immobilised AAVS1 target DNA of Cas9:tracrRNA complexed with crRNA (red), crHyb (black), crDNA (blue), crR.Hyb (green) or crD.Hyb (purple). Shaded areas show SD of three independent measurements. c SwitchSENSE sensogram showing association phase of Cas9:tracrRNA:crXNA complexes binding to target DNA. Shaded areas show SD of four independent measurements