Table 1.

Split tracr fragments sequences

Split tracr fragment	Sequence. RNA in lowercase and DNA in uppercase	DNA/RNA	Design principal
tracr5′ RNA	caaaacagcauagcaaguuaaaauaaggcuaguccguuaucaacuugccuaguggcc
tracr5′ hyb v1	CAAAACAGCATAGCAAguuAaAATAAGGCTAgTCCguuaTCaACTTGCCTAGTGGCC	47/10	Structure-guided design
tracr5′ hyb v2	caaaacagcauagcAAguuAaAATAAGGCTAgTCCguuaTCaACTTGCCTAGTGGCC	33/24	5′-anti-repeat as RNA
tracr5′ hyb v3	CAAAACAGCATAGCaaguuaaaauaaggcuaguccguuaucaacuugccuaguggcc	14/43	Stem loop 1 & 2 as RNA
tracr5′ hyb v4	CAAAACAGCATAGCaaguuaaaauaaggcuaguccguuaucaaCTTGCCTAGTGGCC	28/29	Stem loop 1 as RNA
tracr5′ hyb v5	CAAAACAGCATAGCaaguuaaaATAAGGCTaguccguuaucaacuugccuaguggcc	22/35	½ stem loop 1 and all stem loop 2 as RNA
tracr5′ hyb v6	CAAAACAGCATAGCAAguuaaaauaaggcuaguccguuaucaaCTTGCCTAGTGGCC	30/27	Scanning modification of v4
tracr5′ hyb v7	CAAAACAGCATAGCaaguuaaaaTAAGGCuaguccguuaucaaCTTGCCTAGTGGCC	34/23
tracr5′ hyb v8	CAAAACAGCATAGCaaguuaaaauaaggcTAguccguuaucaaCTTGCCTAGTGGCC	30/27
tracr5′ hyb v9	CAAAACAGCATAGCaaguuaaaauaaggcuagTCCguuaucaaCTTGCCTAGTGGCC	31/26
tracr5′ hyb v10	CAAAACAGCATAGCaaguuaaaauaaggcuaguccguuaTCaaCTTGCCTAGTGGCC	30/27
tracr3′ RNA	ggccactaggcaagtggcaccgagtcggtgcuuuuu
tracr3′ hyb	GGCCACTAGGCAAgTgGCACCgAGTCGGTGcTTTTT	36/4
tracr3′ DNA	GGCCACTAGGCAAGTGGCACCGAGTCGGTGCTTTTT	40/0
tracrHyb.2	GTTGGAACCATTCAAAACAGCATAGCaaguuaaaauaaggcuaguccguuaucaaCTTGAAAAAgTgGCACCgAGTCGGTGcTTTTT	54/33