Figure 1.

Human immune cells express and regulate iodide transporters, thyroglobulin, and thyroid peroxidase. (A) Leukocytes were left unstimulated (US) (−) or activated (+) with PMA and ionomycin for 18 h, total RNA was extracted, and then the cDNA from samples was amplified with PCR. Shown is DNA gel electrophoresis image of two representative donors. (B) Leukocytes mRNA was amplified using real-time quantitative PCR, and then each gene was normalized to internal B2M control. Fold increase over US controls was graphed ± SEM of nine independent donors. (C) Leukocytes were stained with no antibody (control), NIS, or PENDRIN antibodies. The cells were then stained with secondary Alexa-647 and primary-conjugated CD45 Krome-orange antibodies. Leukocyte subsets (granulocytes, monocytes, and lymphocytes) were gated on based on CD45 and side-scatter characteristics. The gated subset histograms are shown as cell counts and antibody staining intensity representative of six donors. (D) Median fluorescent intensity (MFI) values from panel (C) were subtracted from background staining and then graphed ± SEM of six to seven independent donors.