Figure 5.

Concentration-response curves to acetylcholine in the absence (A) or presence of L-NAME (3 × 10⁻⁴ M) plus Indo (10⁻⁵ M) (B) or L-NAME plus Indo plus the IK_Ca and BK_Ca channel blocker charybdotoxin (Charybd; 100 nM) plus the specific SK_Ca channel blocker apamin (Apa; 100 nM) (C) in ascending aorta from wild-type and Marfan syndrome male (left) and female (right) mice. Results are the mean ± SE from wild-type (male n = 4–6; female n = 5–6) and Marfan syndrome (male n = 5–6; female n = 5–6) mice. *P < 0.05, **P < 0.01, ***P < 0.001 by two-way ANOVA.