Table 2.
Putative identification by 16S rRNA gene sequencing of isolates from ready‐to‐eat mixed salad picked from the countable plate of the total aerobic plate (TSA) and Enterobacteriaceae plate (VRBD)
| Closest type straina | Similarity (%) | TSAb | VRBDc |
|---|---|---|---|
| Ready‐to‐eat mixed salad | |||
| Bacillus anthracis (T) AE016877 | 100.0 | 1e | — |
| Bacillus cereus (T) AB190217 | 100.0 | 1d | 1e |
| Bacillus methylotrophicus (T) EU194897 | 96.8 | — | 1e |
| Brevundimonas vesicularis (T) AJ227780 | 100.0 | 1e | — |
| Curtobacterium herbarum (T) AJ310413 | 99.7 | 1d | — |
| Erwinia persicina (T) U80205 | 100.0 | 1e | — |
| Micrococcus luteus (T) AJ536198 | 98.4 | 1e | — |
| Pantoea agglomerans (T) AJ233423 | 98.0 | 1d | 1d |
| Pantoea eucalypti (T) EF688009 | 98.2 | — | 3d+3e |
| Pantoea vagans (T) EF688012 | 100.0 | — | 1d+2e |
| Pseudomonas azotoformans (T) D84009 | 99.8 | 1e | — |
| Pseudomonas constantinii (T) HAMBI 2444 | 100.0 | 1d | — |
| Pseudomonas gessardii (T) AF074384 | 99.8 | 1e | — |
| Pseudomonas grimontii (T) AF268029 | 100.0 | 1e | — |
| Pseudomonas koreensis (T) AF468452 | 100.0 | 1d | — |
| Pseudomonas libanensis (T) AF057645 | 100.0 | 1d | — |
| Pseudomonas marginalis (T) Z76663 | 100.0 | 1e | — |
| Pseudomonas psychrophila (T) AB041885 | 99.7 | 1d+1e | — |
| Rahnella aquatilis (T) AJ233426 | 99.9 | 2d | — |
| Serratia ficaria (T) AJ233428 | 94.3 | — | 1d |
| Serratia liquefaciens (T) AJ306725 | 99.7 | — | 1e |
| Shewanella putrefaciens (T) X81623 | 100.0 | 1d+1e | — |
| Staphylococcus warneri (T) L37603 | 97.8 | — | 1e |
| Yersinia aldovae (T) AF366376 | 99.6 | 1e | — |
| Romaine lettuce | |||
| Arthrobacter polychromogenes (T) X80741 | 99.5 | 2d | — |
| Bacillus anthracis (T) AB190217 | 99.4 | 1d | 1e |
| Bacillus cereus (T) AE016877 | 100.0 | 1e | — |
| Bacillus safensis (T) AF34854 | 100.0 | 1e | — |
| Microbacterium phyllosphaerae (T) AJ277840 | 99.8 | 1e | — |
| Pantoea agglomerans (T) AJ233423 | 98.1 | 2d | 2e |
| Pantoea eucalypti (T) EF688009 | 96.0 | 1d | 1d |
| Pantoea vagans (T) EF688012 | 97.0 | 1e | — |
| Pseudomonas frederiksbergensis (T) AJ249382 | 99.9 | — | 1d |
| Pseudomonas koreensis (T) AF468452 | 99.8 | 1d | — |
| Staphylococcus warneri (T) L37603 | 100.0 | — | 1e |
| Stenotrophomonas chelatiphaga (T) EU573216 | 99.7 | 1d | — |
a
Closest type strain (T) according to RDP data base, Seqmatch software(5).
b
Number of isolates from TSA.
c
Number of isolates from VRBD.
d
isolate found before washing (unwashed).
e
isolate found after washing (washed).