Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2017 Oct 2;149(10):921–934. doi: 10.1085/jgp.201711842

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2017 Bohannon et al.

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms/). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 International license, as described at https://creativecommons.org/licenses/by-nc-sa/4.0/).

PMC Copyright notice

Figure 8. — tPA slows fusion pore expansion in the presence of NPY-pHl. Bovine chromaffin cells were cotransfected with either NPY-pHl and pcDNA3 or NPY-pHl and tPA. pTIRF microscopy was performed as described in Materials and Methods. (A and C) Changes in pHl fluorescence were recorded over time. (B and D) Concurrently, DiD fluorescence as excited by P- and S-polarized light was recorded. The ratio P/S, plotted against time, corresponds to localized increases in membrane curvature. Secretion start time is indicated by the dotted red line. (E) Increases in P/S are reported semiquantitatively. The length of time P/S was elevated was measured for n = 22 NPY-pHl + pcDNA3 or n = 33 NPY-pHl = tPA events and binned as shown.