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. 2017 Jan 5;36(4):487–502. doi: 10.15252/embj.201695050

Figure EV3. Lipid droplet and phospholipid content of ACC1 uORF mutant cells in the cell cycle.

Figure EV3

  1. Representative images to visualize neutral lipids using fluorescence microscopy of wild‐type (uORF + ‐ACC1) cells stained with Nile Red as described in Materials and Methods. The images were taken from cells grown in rich, glucose‐containing medium at the following states: proliferating exponentially (Log); arrested in mitosis with nocodazole (M); and 20 or 40 min after they were released from the arrest (20′ or 40′).
  2. Images of ACC1 uORF mutant cells (TG‐340AA‐ACC1), obtained as in (A).
  3. Quantification of the lipid droplet (Neutral Lipids) staining from (A and B), displayed with box‐plots, normalized for the values of the “Log uORF + ‐ACC1” sample. The median and the inter‐quartile range are indicated, along with the number of cells examined in each case. The whiskers extend to 1.5 times the interquartile range.
  4. Strip charts showing the quantification of total phospholipid (PLs) content from the same cell populations shown in (A–C), normalized for the values of the “Log uORF + ‐ACC1” sample.
Data information: In all microscope images, 5 μm scale bars are indicated.