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A
TEAD luciferase reporter assay in 293T cells expressing MRTFA/B and/or YAP (n = 3). Note that the TEAD luciferase reporter construct lacks any putative SRF‐binding DNA motif.
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B
Genes up‐regulated by either YAP or MRTF over‐expression in the RNA sequencing results.
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C
GSEA for MCF‐10A cells over‐expressing a control or constitutively active MRTF.
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D
Heatmap of the RNA sequencing results for MCF‐10A cells over‐expressing either hyperactive YAP or MRTF. Data are presented as fold change relative to average expression level of control cells in Log2.
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E
qPCR analysis of representative TEAD‐YAP target genes in MCF‐10A cells over‐expressing the indicated genes (n = 3).
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F, G
Western blots from MCF‐10A cells over‐expressing YAP and treated with shRNAs against (F) MRTFA or (G) MRTFB. Asterisk indicates non‐specific band.
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H
qPCR analysis of representative TEAD‐YAP target genes in MCF‐10A cells infected with the indicated viruses (n = 3).
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I
TEAD‐YAP activity reporter (TBS‐luc) and SRF‐MRTF activity reporter (SRF‐RE‐luc) luciferase assay with 293T cells expressing MRTFB and shRNA against SRF (n = 3).
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J
qPCR analysis of CTGF expression in MCF‐10A cells treated with a control guide RNA (sgCAG) or a guide RNA against the SRF‐binding region of the CTGF promoter and infected with the indicated viruses (n = 3).
Data information: Data are presented as means ± s.e.m. *
‐test.
