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. 2016 Dec 22;36(4):475–486. doi: 10.15252/embj.201695959

Figure EV4. The hidden breaks of the 23S rRNA .

Figure EV4

  • A
    Analysis of ribosomal RNA by agarose gel electrophoresis. RNA was extracted from chloroplast 70S ribosome sample and separated on a 2% (w/v) agarose gel (L: high range RNA ladder; 70S: RNA of chloroplast 70S sample).
  • B
    Schematic of rRNA processing and assembly in the chloroplast. Because of two specific cleavage sites on the 23S rRNA, called “hidden breaks”, the 23S rRNA gets separated into three fragments: A (0.5 kb), B (1.2 kb) and C (1.1 kb).
  • C–E
    Views of the 30S (C) and the 50S subunits (D) from the subunit interface and of the 50S subunit (E) from the solvent accessible side. The rRNA is shown as spheres and coloured according to the elements indicated in panel (B). The positions of the hidden breaks on the 23S rRNA are marked with triangles.
  • F–H
    The hidden break indicated with a triangle between fragments A and B is introduced in the connection between helices H2 and H24.
  • I–K
    The hidden break indicated with a triangle between fragments B and C is positioned at the stem loop of helix H63. The binding site of the helicase RH39 on helix H62 is coloured black. The electron density map shown in panels (H and K) is low‐pass filtered to 4 Å, and the nucleotides at the hidden break sites are labelled. The exact positions of the hidden breaks on the 23S rRNA sequence shown in panels (F and I) were stated in a previous publication (Liu et al, 2015).