Figure 5.

Apt-DOX treatment enhanced apoptosis and inhibited cell proliferation in HT29 xenograft tumour. NOD/SCID mice bearing HT29 xenograft tumours with a volume of 50 mm³ were treated with agents as indicated. (A-B) Aptamer-guided DOX delivery inhibited tumour growth and extended survival rate of mice-bearing HT29 tumours. NOD/SCID mice-bearing HT29 xenograft tumour were randomized into six groups and treated as described in the legend. (A) The change of the tumour volume over 63 days (n=4). (B) Kaplan-Meier survival curves of mice (n=4) bearing HT29 tumour treated as indicated. (C) Quantification of apoptotic cells in the treated xenograft tumours using TUNEL assay. (D) Quantification of Ki-67 positive cells in HT29 xenograft tumours treated as indicated. Data shown are means ± SEM. (n=3, unless indicated otherwise). ** P < 0.01 compared with mice receiving free DOX (two-tailed Student's t-test).