Figure 6.

Impairment of CSC self-renewal capability in xenograft tumours following Apt-DOX treatment in vivo. (A-C) Apt-DOX was delivered into EpCAM⁺/CD44⁺/CD24⁺ cells in treated xenograft tumours and eliminated CSCs. (A) Representative micrographs showing efficient targeting of Apt-DOX to EpCAM⁺CD24⁺CD44⁺ cells in HT29 xenograft tumours. Scale bar is 10 μm. (B) Retention of DOX in FACS-sorted EpCAM⁺CD24⁺CD44⁺ cells in xenograft tumours after indicated treatments. The tumour-bearing animals were euthanized 3 h after the last treatment. (C) Quantification of apoptotic EpCAM⁺CD24⁺CD44⁺ cells after various treatments using TUNEL assay (n=4). Data shown are means ± SEM. (n=3, unless indicated otherwise). P value was obtained by the two-tailed Student's t-test.