Figure 4.

Effect of photo-thermal treatment on tumor oxygenation. (A) PA images of 4T1 tumors after different treatments by measuring signals at 750 nm and 850 nm representative of deoxygenated hemoglobin and oxygenated hemoglobin (B) Average total sO₂ of tumor measured over time (C) Representative images of immunofluorescent staining of HIF-1 from 4T1 tumors after treatment with saline/Bi₂S₃+NIR (positive expression: bright green regions). Histogram: quantification of HIF-1, determined from classified images (n = 3). Error bars represent the standard deviation of the mean. P values were calculated by the student's test: ***p < 0.001.