Figure 5.
Effect of photo-thermal treatment on DNA repair. (A) Representative images of immunofluorescent staining of PARP (upper panels) and rad 51 (lower panels) in continuous sections from 4T1 tumors after treatment with saline/Bi2S3+NIR (positive expression: bright green regions. Scale bar corresponds to 100 μm. Histograms: quantification of PARP and rad 51 determined from classified images (n = 3). Error bars represent standard deviation of the mean. P values were calculated by the student's test: *p < 0.05, ***p < 0.001. (B) Representative images of γH2AX immunofluorescent staining of 4T1 cells treated with saline/Bi2S3+NIR (γ-H2AX foci are shown as red fluorescence, cell nuclei stained with Hoechst shown as blue fluorescence). Histogram: quantitation of γ-H2AX immunofluorescent foci formation determined from classified images (n = 3). Error bars represent the standard deviation of the mean. P values were calculated by the student's test: ****p < 0.0001.