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. 2017 Sep 21;7(17):4099–4117. doi: 10.7150/thno.20730

Figure 5.

Figure 5

Effect of isotretinoin (INN) on tumor-derived endothelial cells. (a) Immunofluorescence images of tissue-engineered tumor models untreated or treated with isotretinoin for 5 days. Representative images of tumor-derived endothelial cells (TECs) double-stained for MYCN (green) and CD31 (red) (n=3); nuclei are stained with DAPI (blue). (b) Analysis of the CD31+ compartment in the TE-NB model before after the treatment. CD31+/MYCN+ staining was performed as in (a) for controls and INN treated samples. Total number of CD31+ cells was counted in 4-8 different fields per sample, with >1000 cells/sample counted for each condition. The percentages of HUVEC cells (CD31+) and TECs (CD31+/MYCN+) are shown. Data are shown as Average ± SD (n=3). (c) Analysis of the MYCN+ compartment in the TE-NB model before and after the treatment. These studies were performed as in (b), and the percentages of NB cells (MYCN+) and TECs (CD31+/MYCN+) in this compartment are shown. (c) Representative Immunohistochemical stainings for Ki67+ (red arrows indicate positive cells) and (d) immufluorescence images for CD31+ (red) and MYCN+ (green) showing proliferating cells forming vessels before and after INN treatment.