Figure 6.

Effect of isotretinoin (INN) on vessel-like structures. (a) Representative image of a vessel-like structure observed in TE-NB controls and (b) INN treated samples by double staining for CD31/PAS. (c) mRNA levels of vessel-like structure related genes in drug treated tumor models (n=3) compared to untreated control tumor models (n=3), by qRT-PCR. Relative endogenous expression of vessel-like structure related genes was normalized to GAPDH and error bars represent standard deviations of relative expression. Statistical significance was determined by the two- tailed Student's t test and no differences between groups were observed.