Figure 9.

Dioscin could postpone the progression of established silicosis. (A) Intervention dosing regimen of dioscin in established silicosis. CS instillation was used to induce silicosis and no treatment was given during the first 10 d. Then, silicosis mice were intragastric gavaged with dioscin (80 mg/kg) or vehicle (n=8 per group) daily. Lungs were harvested at the indicated time points for HYP measurement and Masson trichrome staining. (B) Effects of dioscin on mice lungs. (C) HYP content in lung tissues from dioscin or vehicle-treated mice were measured at 28, 42, and 56 d (n = 4-5 per group; *, P<0.05; **, P<0.01). (D) Masson trichrome staining of collagen on lung sections taken from mice at 28, 42 and 56 d after CS instillation. Representative images are shown (n=4-5 per group). Scale bar indicates 200 μm. (E) Fibrotic score analysis of the lung sections of 56 d. The fibrotic area is presented as a percentage. **, P<0.01. Horizontal lines indicate mean.