Skip to main content
. 2017 Sep 26;7(17):4276–4288. doi: 10.7150/thno.20999

Figure 4.

Figure 4

M2 macrophages promote tumor relapse and metastasis after multiple doses of CTX treatment (CTX-M; 75 mg/kg, every 3 days, four times). (A) Schematic illustration of CTX treatment and adoptive M2 macrophage (Mɸ) transfer. (B) Post-CTX-M treatment 4T1 tumor regrowth after M2 macrophage transplantation (Control, n = 8; CTX-M, n = 5; CTX-M + M2 Mɸ, n = 5). (C) Immunofluorescence staining of Ki67 in 4T1 tumor tissues after the indicated treatments (on day 20). Scale, 50 μm. (D) Quantitative analysis of Ki67-positive cells shown in panel C. (E-G) Representative images from India-ink filled lungs (E; scale, 1 cm), quantitative analysis of metastatic lesions (n = 5 per group) (F), and H&E staining (G; scale, 100 μm) of lung sections of 4T1 tumor-bearing mice after the indicated treatments (on day 24 for control group and day 32 for CTX-treated groups). Metastatic lesions are indicated with arrows and dashed circles. *, P <0.05; **, P <0.01; ***, P <0.001.