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. 2017 Sep 26;7(17):4276–4288. doi: 10.7150/thno.20999

Figure 5.

Figure 5

Detection of tumor lymphatic metastases by NIRF and SPECT/CT imaging of M2 macrophages. (A) Schematic illustration of 4T1 lymph node metastasis imaging experiments. (B) BLI results of 4T1 lymph node metastasis model (on days 7, 14, and 21). (C) Representative in vivo NIRF images and ex vivo images after surgery of 4T1 lymph node metastasis 24 h after Dye-αCD206 or Dye-IgG injection. Popliteal lymph nodes are indicated with arrows. (D) Quantified right lymph node (LN)-to-left LN uptake ratios of Dye-αCD206 (n = 4) and Dye-IgG (n = 3). (E) SPECT/CT of 4T1 lymph node metastasis before and after surgery 24 h after 125I-αCD206 injection. Popliteal lymph nodes are indicated with circles. (F) Histological analysis of H&E staining (scale, 100 μm) and immunofluorescence staining (scale, 50 μm) of CD206 in bilateral popliteal lymph node sections from 4T1 lymph node metastatic mice. *, P <0.05.