Table 4.
Comparison of total number virus particles counted by different methods
| PR/8/34 virus | Experiment | EID50 (Log10) | PFU (Log10) | qRT-PCR (Log10) | FFF- MALS (Log10) |
|---|---|---|---|---|---|
| Purified (5 μg/ml) | 1 | 7.3 | 7 | 9.4 | 8.2 |
| 2 | 7.5 | 7.2 | 9.3 | 8.2 | |
| 3 | 7.3 | 7.6 | 8.9 | 8.4 | |
| Mean ± SD | 7.4 ± 0.1 | 7.3 ± 0.3 | 9.2 ± 0.3 | 8.3 ± 0.1 | |
|
| |||||
| Allantoic fluids of infected eggs (1:10 dilution with PBS) | 1 | 7.1 | 7.2 | 8.9 | 8.1 |
| 2 | 7.1 | 7.4 | 8.9 | 8.1 | |
| 3 | 7.3 | 7.2 | 9.9 | 8.3 | |
| Mean ± SD | 7.2 ± 0.1 | 7.3 ± 0.1 | 9.2 ± 0.6 | 8.2 ± 0.1 | |
|
| |||||
| Supernatant from infected MDCK cells (undiluted) | 1 | 7.5 | 7.3 | 10.3 | 8.5 |
| 2 | 7.5 | 7.1 | 9.8 | 8.6 | |
| 3 | 7.8 | 7.4 | 9.6 | 8.7 | |
| Mean ± SD | 7.6 ± 0.2 | 7.3 ± 0.2 | 9.9 ± 0.4 | 8.6 ± 0.1 | |
|
| |||||
| Purified, BPL treated (5 μg/ml) | 1 | N/Aa | N/A | 8.5 | 8.2 |
| 2 | N/A | N/A | 8.7 | 8.3 | |
| 3 | N/A | N/A | 8.9 | 8.3 | |
| Mean ± SD | 8.7 ± 0.2 | 8.3 ± 0.06 | |||
|
| |||||
| Purified, BPL treated RGA/Indonezia/5/05 (1.5 μg/ml) | 1 | N/A | N/A | 8.1 | 7.4 |
| 2 | N/A | N/A | 8.5 | 7.3 | |
| 3 | N/A | N/A | 8.1 | 7.2 | |
| Mean ± SD | 8.2 ± 0.2 | 7.3 ± 0.1 | |||
a
N/A-Not applicable