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. Author manuscript; available in PMC: 2017 Nov 20.
Published in final edited form as: Diabetes Res (Fairfax). 2015 Aug 21;1(4):84–91. doi: 10.17140/DROJ-1-115

Figure 2.

Figure 2

Apelin gene therapy on myocardial ROS formation. (A) apelin gene therapy significantly reduced gp91phox expression compared to WT-STZ mice treated with Ad-β-gal. Knockout of Sirt3 blunted apelin gene therapy-mediated gp91phox expression in Sirt3 KO-STZ mice (n=3 mice, †P<0.001). (B and C) DHE staining of ROS formation shows that Ad-apelin treatment dramatically inhibited ROS formation in the heart of WT-STZ mice (n=5, †P<0.001). Ad-apelin treatment had little effect on the ROS formation level in Sirt3 KO-STZ mice. WT-STZ mice treated with Ad-apelin had a lower ROS formation level than Sirt3KO-STZ, but did not reach significant difference. The ROS formation levels were similar between mice received Ad-apelin and Ad-β-gal treatment in Sirt3KO-STZ mice.