Figure 4.

Promoters determine XRN2 dependence in transcription termination. (A) A transcription readthrough reporter. The indicated construct was integrated into an intergenic region with no active transcription. (B–G) Animals carrying the indicated constructs were treated with mock or xrn-2 RNAi from L1 to L4 stage and observed for GFP signal. The insets show differential interference contrast (DIC) images that confirm mid-L4 stage vulva morphology. Bar, 100 µm. (H) Summary of the reporter assay. All phenotypes, GFP-positive (+) or GFP-negative (−), were fully penetrant and scored for ≥10 animals per condition.