FIG 2.

Bmp gene expression is not increased, but activin B (Inhbb) gene expression increases with hepcidin gene expression in Plasmodium berghei-infected BALB/c mice. (A) Liver Bmp6 mRNA expression decreases on day 8 of infection (n = 3 mice per day per experiment, n = 9 total). (B to D) A representative experiment (n = 3 mice per day) was examined further to see if other Bmp genes increase in other candidate tissues. (B) Bmp6 mRNA did not show any trend toward upregulation in spleen or bone marrow. (C) Bmp2 did not increase in liver, spleen, or bone marrow. (D) Bmp9 mRNA was undetectable in spleen and bone marrow and did not increase in liver. Hepatic activin B message (Inhbb) is increased on day 8 postinfection (E), while hepatic activin A mRNA (Inhba) is decreased (F). Inhbb expression shows a trend toward correlation with Hamp1 (G) and correlates with Id1 (H). Statistical analyses in dot plots are Dunn's multiple-comparison tests after Kruskal-Wallis test. *, P < 0.05; **, P < 0.01; ****, P < 0.0001. All correlations are from Spearman's correlation tests.