FIG 2.
Effects of sse and sagA deletions on skin invasion, GAS loads in liver and spleen, and neutrophil recruitment in subcutaneous MGAS315 infection of C57BL/6J mice. Six-week old C57BL/6J mice were subcutaneously inoculated with 1.5 × 108 CFU of MGAS315, 1.9 ×108 CFU of the Δsse mutant, 1.8 × 108 CFU of the ΔsagA mutant, or 1.7 × 108 CFU of the Δsse ΔsagA mutant and euthanized at 24 h after inoculation for analyses. Shown are the lesion size (A), neutrophil recruitment (B), GAS loads in liver (C), and GAS loads in spleen (D). P values were determined by the Mann-Whitney t test. *, P < 0.05; **, P < 0.01; ns, not significant.